Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910. Ethidium bromid e summary of data for chenflcal selection. It fluoresces a redorange colour under ultraviolet light and with increased fluorescence when bound to doublestranded dna. Ultrapure ethidium bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and caesium chloride cscl gradients. Ethidium bromide, 10mgml aqueous solution safety data sheet according to federal register vol. Well, we finally standardized gelred dye and we want to decontaminate all the working area.
Ethidium bromide 2,7diamino10ethyl9phenylphenanthridinium bromide is used as a nucleic acid stain which fluoresces in the presence of ultraviolet uv light. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and 25fold on binding doublestranded dna so that destaining the background is not necessary with a low stain concentration 10. Sds ethidium bromide page 7 of 8 other adverse effects no data available product dispose of unused product, residues, and containers according to local, regional, state, and national regulations.
In case of skin contact immediately wash off with soap and plenty of water. Disposal of electrophoresis buffer solutions and gels overview. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Etbr is a dark red, crystalline, nonvolatile solid, moderately soluble in water, which fluoresces. Remove person to fresh air and keep comfortable for breathing. How to decontaminate the working zone with ethidium bromide. Ethidium bromide etbr is the most commonly used nucleic acid stain for page or agarose gel electrophoresis. Feb 27, 2020 ethidium bromide is a potent mutagen which has been commonly used as a nucleic acid stain for many years. One gml ethidium bromide was chosen in the preceding experiments because it is at the high limit of concentrations used to stain agarose gels for rna. Ethidium bromide laboratory spill and waste handling procedures. The powder is used to make a stock solution of 10 mgml, and the working concentration for gelstaining is 1 gml. Are other fluorescent tags used instead of ethidium bromide. This policy provides general guidance to researchers and labs on how to work safely with ethidium bromide. The user must determine suitability of this information for his application.
Promega corporation 2800 woods hollow road madison, wi 53711 u. Mixture name product identifier % classification ghsus water, reagent grade, usp purified cas no 7732185 98 not classified. The filtrate may be poured down the drain the used filters are disposed of similar to gels. Ethidium bromide solution 10 mgml catalog or product number. The mechanism of action for such compounds is known, in which these compounds are able to bind to the kinetoplastid dna and to alter their conformation to zdna molecules that stop replication of. Ethidium bromide is an intercalating agent commonly used as a fluorescent tag nucleic acid stain in molecular biology laboratories for techniques such as agarose gel electrophoresis. Ethidium bromide solution sc286960 hazard alert code key. The crystal or powder form is odorless and appears dark red in color. Ethidium bromide is a powerful mutagen and is a skin, eye and respiratory irritant. Identification of the substancemixture and of the companyundertaking 1. In case of contact, immediately flush skin with plenty of water. Ethidium bromide is a potent mutagen used as a nucleic acid stain.
No producers of eb were reported in epas 1983 tscapp database cis, 1994. Ethidium bromide etbr is commonly used as a nonradioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other methods of nucleic acid separation. Chemical product and company identification product name. Ask most biologists about handling it, and youre get a fearful expression and advice to use gloves, etc. Ethidium bromide is typically purchased in powder or solution form and is soluble in water. Filtering aqueous ethidium bromide waste solutions that are free of other contaminants through a bed of activated charcoal is a relatively simple and effective method for removal of ethidium bromide. Exposure information commercial availabili production and producers. Correlation of neurologic symptoms with blood levels of bromide is inexact. Ethidium bromide cas no 1239458 material safety data sheet sds msds section 1. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet light, providing molecular biologists with an excellent stain for visualizing and imaging nucleic acids. Due to its unique structure, it can easily intercalate into dna strand.
Eye protection personnel handling ethidium bromide must wear adequate eye protection. Material safety data sheet pippin prep replacement reagent kits. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet. It is commonly sold in a powder form which is soluble in water. Ethidium bromide laboratory safety environmental health. How to decontaminate the working zone with ethidium bromide hello dear friends. Pippin preptm precast agarose gel cassettes with buffer solution product numbers. Ethidium bromide is typically in powder or solution form and is soluble in water. Product ethidium bromide solution, 10ml product code h5041 substance number description amount symbols h504 ethidium bromide solution 1 t.
No warranty, express or implied, is made and labchem inc. Bp210 synonyms no information available recommended use laboratory chemicals. Not intended for human or animal diagnostic or therapeutic uses. Typically eb is purchased in powder or solution form and is soluble in water. Ethidium bromide etbr, a dark red, crystalline, nonvolatile powder that is moderately soluble in water, is widely used in research laboratories as a visualizing agent for nucleic acids. Eb was reported by watkins 1952 to be prepared from 3. Material safety data sheet section 1 ethidium bromide 1 chemical product and company identification msds name. Tufts university standard operating procedure sop for. Dot us not dangerous goods imdg not dangerous goods. Product name ethidium bromide 10mgml page 1 7 for research use only. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the usa. Do not dispose of ethidium bromide as biohazardous waste. For agarose gel cassettes with ethidium bromide dye dna version.
Material safety data sheet ethidium bromide solution. Contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide staining are not required. Sdsethidium bromide page 3 of 8 if inhaled if breathed in, move person into fresh air. Thats because the compound is used to make dna fluoresce when running gels, and it does that by slipping neatly betw. Ethidium bromide etbr is commonly used as a nonradioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other. Ethidium bromide is a toxic chemical and a mutagen. A safer alternative is to purchase ethidium bromide in solution. Ethidium bromide waste disposal fact sheet environmental. A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. The detection limit of dna bound to ethidium bromide.
P501 dispose of contentscontainer to hazardous or special waste. Contaminated packaging dispose of as unused product. Dispose of the green bag in the biohazardous waste box for incineration. Ethidium bromide, 1% solution molecular biology revision date 18jan2018 sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590 nm. Contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract.
Review the material safety data sheet msds prior to using this material. Companyundertaking identification product name 17jul2014 ethidium bromide. Ethidium bromide has mutagenic properties that may present a hazard if it is poured down the drain untreated or placed in the trash. Wipe the area with a damp cloth after the work with ethidium bromide powder is complete. Please follow the instructions listed in this fact sheet when handling etbr.
It fluorescents a redorange color under ultraviolet uv light and with increased fluorescence when bound to doublestranded dna. Ethidium bromide etbr is a flat molecule that fits between adjacent base pairs. In the case of etbr ingestion, obtain medical attention. Fisher scientific 1 reagent lane fair lawn, nj 07410. Ethidium bromide 3,8diamino5ethyl6 phenylphenanthridinium bromide is the classic dna stain. Thermo scientific pierce ethidium bromide is available as a dilute 0. Accidental release measures personal precautions wear respiratory protection. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes.
The fluorescence of etbr increases 21fold upon binding to doublestranded rna. Dec 19, 20 ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. Homidium bromide nfpa 4, hmis 3 toxicity ethidium bromide etbr is commonly used in molecular biology laboratories for visualizing nucleic acids using electrophoresis and other gelbased nucleic acid separation methods. Ethidium bromide is a dna intercalator, inserting itself between the base pairs in the double helix. Ethidium bromide is commonly used in molecular biology laboratories for dna identification in electrophoresis. Ethidium bromide ethidium bromide is an intercalating agent which resembles a dna base pair. It has a role as an intercalator and a fluorochrome.
Rna sample loading buffer, with etbr ethidium bromide, 50gml is formulated for use in rna sample preparation for denaturing formaldehydeagarose gel electrophoresis. Ethidium bromide ethidium bromide 2,7diamino10ethyl9phenylphenanthridinium bromide etbr remains a widely. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as provided for csc1 gradient protocols. Ethidium bromide etbr wastes are not regulated by the state of massachusetts or the epa. Ethidium bromide is used to visualize dna in agarose gels and in many cesium chloride gradient protocols. Material safety data sheet ethidium bromide solution sdsmsds. Ethidium bromide is a potent mutagen, often used in laboratories to stain nucleic acids in electrophoresis gels. Singlestranded rna can also be detected, since it usually folds back onto itself and thus provides local base pairing for the dye to intercalate. Apr 18, 2016 ethidium bromide is found in pretty much every molecular biology lab around. Wear gloves and a face mask when working with ethidium bromide in powdered form. Place the green bag into the ethidium bromide solution. Electrophoresis gels are commonly used in molecular biology laboratories for the identification of dna and proteins.
Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards, precautions should be taken during handling and disposal. Ethidium is the fluorescent compound widely used in experimental cell biology and biochemistry to reveal doublestranded dna and rna. Identification product name ethidium bromide, 1% solutionmolecular biology cat no. Sodium bromide 6 information in this msds is from available published sources and is believed to be accurate. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet. It is available as an aqueous solution containing 10 mgml ultrapure ethidium bromide and highpurity water.
Ethidium bromide safe handling and disposal procedures. Rna sample loading buffer, with etbr scbt santa cruz. If the pdf does not display below, you may also download it here. Find msds or sds, a coa, data sheets and more information. Page 18 material safety data sheet university of lausanne.
The next experiment was designed to determine the optimal concentration of ethidium bromide. Ethidium bromide is strongly mutagenic, causing living cell mutations. The toxic effects of etbr may be experienced if swallowed, inhaled, or absorbed through the skin. Each drop contains 25 g of ethidium bromide and is ideal for a 50 ml agarose gel after. Disposal, decontamination, and destruction procedure. The powder form is considered an irritant to the upper respiratory track, eyes and skin. Ethidium bromide safe handling, disposal, and emergency procedures.
Fisher scientific 1 reagent lane fair lawn, nj 07410 for information, call. Safety data sheet creation date 08jun2010 revision date 18jan2018 revision number 4 1. H504 application of the substance the mixturelaboratory chemicals details of the supplier of the safety data sheet manufacturersupplier. The resource to the url or file is currently unavailable rated 5 out of 5 by kg from various publications cite the use of ethidium various publications cite the use of ethidium bormide, an intercalating fluorescing small molecule, for staining of ribonucleic acids. Yes you should, otherwise this misinformation is detrimental to the world, environmental effects of massive scale agriculture, and economies. Product name ethidium bromide, 1% solutionmolecular biology cat no. Sigmaaldrich 3050 spruce street saint louis mo 63103 usa telephone. Protocol for rna quantitation using ethidium bromide etbr. Ethidium bromide solution material safety data sheet sds msds. Ethidium bromide is commonly used to detect nucleic acids in molecular biology laboratories. In the case of dna this is usually doublestranded dna from pcrs, restriction digests, etc. Chemical product and company identification section 1.